mouse anti cdk6 monoclonal antibody Search Results


cdk6 antibodies  (R&D Systems)
89
R&D Systems cdk6 antibodies
Cdk6 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk6  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc cdk6
Cdk6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk6  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology cdk6
Figure 6 Protein expression of cyclin D1, cyclin D2, cyclin D3, cdk2, cdk4, and <t>cdk6</t> in cultured MBEC and human ICC cells (CCKS1, HuCCT1, HuH28) with and without TGF-b1 treatment (3.0 ng/ml, 48 h). TGF-b1 inhibits expression of cyclin D1, cdk4 and cdk6 in MBEC. TGF-b1 also inhibits expression of cdk4 (CCKS1, HuCCT1) and cdk6 (CCKS1, HuH28) in ICC cells, while cyclin D1 expression is not influenced by TGF-b1 treatment in ICC cells.
Cdk6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdk6/product/Santa Cruz Biotechnology
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mouse cdk6 antibody  (Proteintech)
93
Proteintech mouse cdk6 antibody
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
Mouse Cdk6 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse cdk6 antibody/product/Proteintech
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cdk6  (Proteintech)
96
Proteintech cdk6
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
Cdk6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti cdk6  (Atlas Antibodies)
92
Atlas Antibodies rabbit anti cdk6
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
Rabbit Anti Cdk6, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal cdk6 antibody  (Danaher Inc)
86
Danaher Inc mouse monoclonal cdk6 antibody
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
Mouse Monoclonal Cdk6 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-cyclin a1 antibody  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology mouse anti-cyclin a1 antibody
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
Mouse Anti Cyclin A1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4656s rabbit monoclonal anti cdk6 cst  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc 4656s rabbit monoclonal anti cdk6 cst
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
4656s Rabbit Monoclonal Anti Cdk6 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk6 sc 177  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology cdk6 sc 177
a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and <t>CDK6</t> ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.
Cdk6 Sc 177, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin d1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc cyclin d1
FIGURE 2 IATL induces G2/M phase cell cycle arrest in melanoma cells. (a) Effects of IATL on cell cycle distribution in melanoma cells. The proportion of cells in different phases was analyzed using flow cytometry. (b) Western blot analyses of G2/M phase cell cycle arrest-related proteins. Proteins levels of CDC2, CDK6, <t>Cyclin</t> <t>D1,</t> and Cyclin B1 were detected by Western blotting. β-Actin was served as a loading control. Left panels, representative Western blotting results; right panels, band intensity results analyzed using ImageJ software. Data from three independent experiments are shown as mean ± SD. **p < 0.01, vs. vehicle control (DMSO-treated group).
Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cdk6 antibody  (Biorbyt)
93
Biorbyt anti cdk6 antibody
FIGURE 2 IATL induces G2/M phase cell cycle arrest in melanoma cells. (a) Effects of IATL on cell cycle distribution in melanoma cells. The proportion of cells in different phases was analyzed using flow cytometry. (b) Western blot analyses of G2/M phase cell cycle arrest-related proteins. Proteins levels of CDC2, CDK6, <t>Cyclin</t> <t>D1,</t> and Cyclin B1 were detected by Western blotting. β-Actin was served as a loading control. Left panels, representative Western blotting results; right panels, band intensity results analyzed using ImageJ software. Data from three independent experiments are shown as mean ± SD. **p < 0.01, vs. vehicle control (DMSO-treated group).
Anti Cdk6 Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6 Protein expression of cyclin D1, cyclin D2, cyclin D3, cdk2, cdk4, and cdk6 in cultured MBEC and human ICC cells (CCKS1, HuCCT1, HuH28) with and without TGF-b1 treatment (3.0 ng/ml, 48 h). TGF-b1 inhibits expression of cyclin D1, cdk4 and cdk6 in MBEC. TGF-b1 also inhibits expression of cdk4 (CCKS1, HuCCT1) and cdk6 (CCKS1, HuH28) in ICC cells, while cyclin D1 expression is not influenced by TGF-b1 treatment in ICC cells.

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Intrahepatic cholangiocarcinoma escapes from growth inhibitory effect of transforming growth factor-beta1 by overexpression of cyclin D1.

doi: 10.1038/labinvest.3700236

Figure Lengend Snippet: Figure 6 Protein expression of cyclin D1, cyclin D2, cyclin D3, cdk2, cdk4, and cdk6 in cultured MBEC and human ICC cells (CCKS1, HuCCT1, HuH28) with and without TGF-b1 treatment (3.0 ng/ml, 48 h). TGF-b1 inhibits expression of cyclin D1, cdk4 and cdk6 in MBEC. TGF-b1 also inhibits expression of cdk4 (CCKS1, HuCCT1) and cdk6 (CCKS1, HuH28) in ICC cells, while cyclin D1 expression is not influenced by TGF-b1 treatment in ICC cells.

Article Snippet: The membranes were incubated with primary antibodies to cyclin D1 (clone HD11, 1:100, mouse monoclonal, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), cyclin D2 (clone M-20, 1:100, rabbit polyclonal, Santa Cruz Biotechnology, Inc.), cyclin D3 (clone D-7, 1:100, mouse monoclonal, Santa Cruz Biotechnology, Inc.), cdk2 (clone H-298, 1:200, rabbit polyclonal, Santa Cruz Biotechnology, Inc.), cdk4 (clone H-303, 1:200, rabbit polyclonal, Santa Cruz Biotechnology, Inc.), cdk6 (clone H-96, 1:200, rabbit polyclonal, Santa Cruz Biotechnology, Inc.), TbR-II (2 mg/ml, goat polyclonal, R&D Systems, Inc.), or b-actin (clone AC-15, 1:5000, mouse polyclonal, Abcam Limited, Cambridge, UK).

Techniques: Expressing, Cell Culture

a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and CDK6 ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.

Journal: Nature Communications

Article Title: DNA framework-engineered chimeras platform enables selectively targeted protein degradation

doi: 10.1038/s41467-023-40244-7

Figure Lengend Snippet: a Schematic illustration of bis-DbTACs design, which is based on DbTACs. b Schematic illustration of three ligand covalent sites of bis-DbTACs equivalent to a DNA tetrahedral scaffold with three polyA domains. Au NPs (5, 10, and 15 nm) correspond to CRBN, CDK9, and CDK6 ligands, respectively. c Cartoon and representative TEM images of bis-DbTACs equivalents. Scale bars are 75 Å and 200 Å, respectively. d WB analysis of the selectively targeted degradation ability of bis-DbTACs at different concentrations and semiquantitative analysis of the grayscale. The error bars indicate the mean ± SD values; n = 3. e Immunofluorescence double-staining images of HepG2 cells treated with/without bis-DbTACs were recorded by confocal laser scanning microscopy. The cell nucleus was stained with DAPI. CDK6 and CDK9 proteins were labeled with anti-CDK6 and anti-CDK9 antibodies, respectively. Scale bars, 10 μm.

Article Snippet: The primary antibody used was mouse CDK6 antibody (Proteintech Group, Rosemont, IL, USA, 66278-1-Ig, 1:100), rabbit CDK9 polyclonal antibody (Proteintech Group, Rosemont, IL, USA, 11705-1-AP, 1:100).

Techniques: Immunofluorescence, Double Staining, Confocal Laser Scanning Microscopy, Staining, Labeling

FIGURE 2 IATL induces G2/M phase cell cycle arrest in melanoma cells. (a) Effects of IATL on cell cycle distribution in melanoma cells. The proportion of cells in different phases was analyzed using flow cytometry. (b) Western blot analyses of G2/M phase cell cycle arrest-related proteins. Proteins levels of CDC2, CDK6, Cyclin D1, and Cyclin B1 were detected by Western blotting. β-Actin was served as a loading control. Left panels, representative Western blotting results; right panels, band intensity results analyzed using ImageJ software. Data from three independent experiments are shown as mean ± SD. **p < 0.01, vs. vehicle control (DMSO-treated group).

Journal: Phytotherapy research : PTR

Article Title: Isoalantolactone exerts anti-melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models.

doi: 10.1002/ptr.8132

Figure Lengend Snippet: FIGURE 2 IATL induces G2/M phase cell cycle arrest in melanoma cells. (a) Effects of IATL on cell cycle distribution in melanoma cells. The proportion of cells in different phases was analyzed using flow cytometry. (b) Western blot analyses of G2/M phase cell cycle arrest-related proteins. Proteins levels of CDC2, CDK6, Cyclin D1, and Cyclin B1 were detected by Western blotting. β-Actin was served as a loading control. Left panels, representative Western blotting results; right panels, band intensity results analyzed using ImageJ software. Data from three independent experiments are shown as mean ± SD. **p < 0.01, vs. vehicle control (DMSO-treated group).

Article Snippet: Primary antibodies against p-PI3K (Tyr458, Tyr199) (#4228), PI3K (#4249), Bcl-2 (#4223), Bax (#41162), STAT3 (#9139), p-STAT3 (Tyr705) (#9145), Src (#2109), p-Src (Tyr416) (#59548), CDC2 (#28439), Cyclin D1 (#55506), CDK6 (#13331), Cyclin B1 (#12231), Mcl-1 (#94296), Bcl-XL (#2764), p-AKT (Ser473) (#4060), AKT (#4691), p-mTOR (Ser2448) (#5536), mTOR (#2983), p-4E-BP1 (Thr37/46) (#39788), 4E-BP1 (#9644), Poly (ADP-Ribose) Polymerase (PARP) (#9532), cleaved-caspase 3 (#9661), and LC3B (#3868) were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Flow Cytometry, Western Blot, Control, Software